Product Information

Contents: Biotin anti-human IL-17A (Interleukin-17A)
Catalog Number: 13-7179
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio64DEC17
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7179	Fluorescein isothiocyanate (FITC) anti-human IL-17A (Interleukin-17A)	488	518	IC Flow
12-7179	Phycoerythrin (PE) anti-human IL-17A (Interleukin-17A, IL17A)	488	575	IC Flow
13-7179	Biotin anti-human IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISA det ELISPOT det
14-7179	Affinity Purified anti-human IL-17A (Interleukin-17A)	N/A	N/A	ELISA IHC(Paraffin)
45-7179	PerCP-Cy5.5 anti-human IL-17A (Interleukin-17A)	488	690	IC Flow
51-7179	Alexa Fluor® 647 anti-human IL-17A (Interleukin-17A, IL17A)	633	668	IC Flow
53-7179	Alexa Fluor® 488 anti-human IL-17A (Interleukin-17A, IL17A)	488	519	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies. Click here for link to data 51-7179.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio64DEC17 antibody has been reported for use as the detection antibody in human IL-17A ELISA and ELISPOT assays, as well as for neutralization.

Applications Tested

The biotinylated eBio64DEC17 antibody has been tested as the detection antibody in a sandwich ELISA for measurement of human IL-17A protein levels, in combination with the Affinity Purified eBio64CAP17 antibody (14-7178) for capture and recombinant human IL-17A (14-8179) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5 – 2.0 ug µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Acosta-Rodriguez EV, Napolitani G, et al. 2007. Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat Immunol. 8(9):942-9. (FC, PubMed)

Chen Z, Tato CM, Muul L, Laurence A, O'Shea JJ. Distinct regulation of interleukin-17 in human T helper lymphocytes. Arthritis Rheum. 2007 Sep;56(9):2936-46. (ebio64Dec17, FC PubMed)

Hirata T, Osuga Y, et al. 2008. Interleukin (IL)-17A Stimulates IL-8 Secretion, Cyclooxygensase-2 Expression, and Cell Proliferation of Endometriotic Stromal Cells. Endocrinology 149(3):1260-7. (eBio64DEC17, IHC paraffin, PubMed)

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