Product Information

Contents: Biotin anti-human interleukin-13 (IL-13)
Catalog Number: 13-7138
Sizes: 50 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Human PBMCs (300,000/well) in 100 ul complete RPMI medium were treated without (left) or with (right) Candida (100ug/mL) for 24 hrs in an IL-13 ELISPOT assay plate (pre-coated with functional grade PVM13-1 antibody).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7138	Biotin anti-human Interleukin (IL-13, IL13) polyclonal	N/A	N/A	ELISA det ELISPOT det

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Description

The polyclonal antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8⁺ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The biotinylated anti-human IL-13 polyclonal antibody has been reported for use in ELISA and ELISPOT.

Applications Tested

The biotinylated anti-human IL-13 polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-13 in combination with the purified PVM13-1 (14-7139) antibody for capture and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant cytokine standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

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