Biotin anti-mouse IL-13 (Interleukin-13, IL13)

Contents: Biotin anti-mouse IL-13 (Interleukin-13)
Catalog Number: 13-7135
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio1316H
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-7135-81	50 ug	$95
13-7135-85	500 ug	$260

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7135	Biotin anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA det ELISPOT det
16-7135	Functional Grade* Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1316H antibody reacts with mouse IL-13. The eBio1316H antibody is a neutralizing antibody. Mouse interleukin-13 (IL-13), originally designated p600, is a 11.6 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. IL-13 was originally identified in 1989 through differential hybridization of cDNA libraries prepared from T helper 1 and T helper 2 cells. IL-13 contains four cysteine residues, which form two intramolecular disulfide bonds. IL-13 shares many activities with IL-4; this relates to a requirement for IL-4Ralpha chain for signal transduction. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of inflammatory cytokine expression. IL-13 induces CD23 expression on B cells and enhances CD72, and expression of class II MHC gene products. Mouse IL-13 does not display the activities on T cells and B cell stimulatory effects which IL-4 exhibits. Mouse IL-13 is active on human and mouse cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio1316H antibody has been reported useful for neutralization, ELISA detection, and ELISPOT detection.

Applications Tested

The biotinylated eBio1316H antibody has been tested as the detection antibody in a sandwich ELISA (and ELISPOT assay) for analysis of mouse interleukin-13 (IL-13) in combination with the purified eBio13A (14-7133) antibody for capture and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25-1.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

For intracellular staining of mouse IL-13, the fluorochrome labelled eBio13A antibody is recommended.

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