Product Information

Contents: Biotin anti-mouse IL-13 (Interleukin-13, IL13)
Catalog Number: 13-7133
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBio13A
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7133	Affinity Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISA cap
16-7133	Functional Grade* Purified anti-mouse IL-13 (Interleukin-13, IL13)	N/A	N/A	ELISPOT cap
12-7133	Phycoerythrin (PE) anti-mouse IL-13 (Interleukin-13, IL13)	488	575	IC Flow
51-7133	Alexa Fluor® 647 anti-mouse IL-13 (Interleukin-13, IL13)	633	668	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.

Applications Tested

The biotinylated eBio13A antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-13 (IL-13) in combination with the purified eBio1316H (14-7135) antibody for capture and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

For intracellular staining of mouse IL-13, the fluorochrome labelled eBio13A antibody is recommended.

References

Miyahara, N., et al. 2004. Contribution of antigen-primed CD8+T cells to the develompent of airwary hyperresponsiveness and inflammation is associated with IL-13. J. Immunol. 172: 2539-2558.

McKenzie AN, Li X, Largaespada DA, Sato A, Kaneda A, Zurawski SM, Doyle EL, Milatovich A, Francke U, Copeland NG, et al. Structural comparison and chromosomal localization of the human and mouse IL-13 genes. J Immunol. 1993 Jun 15;150(12):5436-44.

Doherty TM, Kastelein R, Menon S, Andrade S, Coffman RL. Modulation of murine macrophage function by IL-13. J Immunol. 1993 Dec 15;151(12):7151-60.

Terabe M, Matsui S, Noben-Trauth N, Chen H, Watson C, Donaldson DD, Carbone DP, Paul WE, Berzofsky JA. NKT cell-mediated repression of tumor immunosurveillance by IL-13 and the IL-4R-STAT6 pathway. Nat Immunol. 2000 Dec;1(6):515-20.

Grunig G, Warnock M, Wakil AE, Venkayya R, Brombacher F, Rennick DM, Sheppard D, Mohrs M, Donaldson DD, Locksley RM, Corry DB. Requirement for IL-13 independently of IL-4 in experimental asthma. Science. 1998 Dec 18;282(5397):2261-3.

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