human IL-12 antibody, Biotin conjugate

Contents: Biotin anti-human interleukin-12 (IL-12) p40/70
Catalog Number: 13-7129
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: C8.6
Isotype: Mouse IgG1, κ

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13-7129-81

50 ug

13-7129-85

500 ug

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7129	Affinity Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	IC Flow NU WB
16-7129	Functional Grade* Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	NU
13-7129	Biotin anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	ELISA det
12-7129	PE anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	488	575	IC Flow
51-7129	Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	633	668	IC Flow
53-7129	Alexa Fluor® 488 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	488	519	IC Flow
48-7129	eFluor^TM 450 anti-human IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40/70 (Pacific Blue® replacement)	405	450	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C8.6 antibody has been reported for use in ELISA.

Applications Tested

The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p70 in combination with the purified B-T21 (13-7128) antibody for capture and recombinant human IL-12 p70 (14-8129) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5 - 2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
The biotinylated C8.6 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-12 p40 monomer in combination with the purified C8.3 (14-7127) antibody for capture and recombinant human IL-12 p40 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 10000 pg/ml - 80 pg/ml should be included in each ELISA plate.

References

Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.
Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.

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