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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis. Applications TestedThe biotinylated C17.8 antibody has been tested as the detection antibody in three different sandwich ELISAs, for measurement of mouse IL-12 p70, mouse IL-23, or mouse IL-12 p40 total. For mouse IL-12 p70 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in sandwich ELISA for analysis of mouse IL-12 p70, in combination with the purified C18.2 antibody (14-7122) for capture and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/ml - 15 pg/ml should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates. For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombinant mouse IL-23 (14-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4,000 pg/ml - 30 pg/ml should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate. For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombinant mouse IL-12 p40 (14-8122), IL-12 p70 (14-8121) or mouse IL-23 (14-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/ml - 15 pg/ml should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates. References
Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34. Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72. Related ProductsCat. 00-0000 eBioscience 10X ELISA Coating Buffer Cat. 44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate Cat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 00-4201 1X eBioscience TMB Substrate Solution Cat. 00-4202 eBioscience ELISA Diluent Solution, 5X Cat. 00-4203 Super AquaBlue ELISA Substrate Cat. 14-7122 Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) (clone C18.2) Cat. 14-7125 Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C15.6) Cat. 88-7231 Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) Cat. 16-7232 Functional Grade Purified anti-mouse IL-23 (Interlekin-23, IL23) p19 (clone G23-8) Cat. 88-7234 Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go! Cat. 88-7921 Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) Cat. 14-8121 Recombinant Mouse IL-12 (Interleukin-12, IL12) p70 Cat. 14-8122 Recombinant Mouse IL-12 (Interleukin-12, IL12) p40 monomer Cat. 14-8123 Recombinant Mouse IL-12 (Interleukin-12, IL12) p40 homodimer Cat. 14-8231 Recombinant Mouse IL-23 (Interleukin-23, IL23) Cat. 34-8231 Carrier-Free Recombinant Mouse IL-23 (Interleukin-23, IL23) |
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