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Anti-Mouse IL-12/IL-23 p40 Biotin

Also known as: Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40

RUO: For Research Use Only
 
Contents: Anti-Mouse IL-12/IL-23 p40 Biotin
Catalog Number: 13-7123
Concentration: 0.5 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 2-8°C. Do not freeze.
Clone: C17.8
Host/Isotype: Rat IgG2a, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
13-7123-81 50 ug
To view relevant pricing, please select your country and resume browsing.

13-7123-85 500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
00-4201 1X TMB ELISA Substrate Solution
00-4202 ELISA Diluent Solution (5X)
00-4203 Super AquaBlue ELISA Substrate
14-7122 Anti-Mouse IL-12 Purified (C18.2)
14-7125 Anti-Mouse IL-12/IL-23 p40 Purified (C15.6)
14-8121 Mouse IL-12 p70 Recombinant Protein
14-8231 Mouse IL-23 Recombinant Protein
16-7232 Anti-Mouse IL-23 p19 Functional Grade Purified (G23-8)
18-4100 Avidin HRP
34-8231 Mouse IL-23 Recombinant Protein Carrier-Free
44-2404 Nunc MaxiSorp® flat-bottom 96 well plate
88-7231 Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
88-7234 Mouse IL-23 ELISA Ready-SET-Go!®
88-7921 Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (See replacement item BMS6004)
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
12-7123 Anti-Mouse IL-12/IL-23 p40 PE 488 575 IC Flow RUO
13-7123 Anti-Mouse IL-12/IL-23 p40 Biotin N/A N/A ELISA det RUO
16-7123 Anti-Mouse IL-12/IL-23 p40 Functional Grade* Purified N/A N/A IC Flow IP NU WB RUO
45-7123 Anti-Mouse IL-12/IL-23 p40 PerCP-Cy5.5 488 690 IC Flow RUO
51-7123 Anti-Mouse IL-12/IL-23 p40 Alexa Fluor® 647 633 668 IC Flow RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.
Applications Tested
The biotinylated C17.8 antibody has been tested as the detection antibody in three different sandwich ELISAs, for measurement of mouse IL-12 p70, mouse IL-23, or mouse IL-12 p40 total.

For mouse IL-12 p70 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in sandwich ELISA for analysis of mouse IL-12 p70, in combination with the purified C18.2 antibody (14-7122) for capture and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/ml - 15 pg/ml should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombinant mouse IL-23 (14-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4,000 pg/ml - 30 pg/ml should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate.

For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombinant mouse IL-12 p40 (14-8122), IL-12 p70 (14-8121) or mouse IL-23 (14-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/ml - 15 pg/ml should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.
References

Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB.
Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34.
Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T.
IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72.

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