Product Information

Contents: Biotin anti-mouse interleukin-1β (IL-1β)
Catalog Number: 13-7112
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rabbit IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7112	Biotin anti-mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA det

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Description

The biotinylated polyclonal rabbit antibody reacts with mouse interleukin-1β (IL-1β), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. IL-1 has effects on B cells, promoting cell proliferation and immunoglobulin synthesis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Biotin anti-mouse interleukin-1β (IL-1β) has been reported for use as the detection antibody for a mouse IL-1β ELISA.

Applications Tested

The biotinylated polyclonal rabbit anti-mouse IL-1β antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-1β in combination with the unlabelled B122 (14-7012) antibody for capture and recombinant mouse IL-1β (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 4.0 - 6.0 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

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