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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe IL-1α polyclonal rabbit antibody reacts with mouse interleukin-1α (IL-1α), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This biotin anti-mouse interleukin-1α (IL-1α) polyclonal has been reported for use in ELISA. Applications TestedThis polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse interleukin-1α (IL-1α) in combination with the purified ALF-161 (14-7011) antibody for capture and recombinant mouse IL-1α (14-8011) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. Related ProductsCat. 00-0000 eBioscience 10X ELISA Coating Buffer Cat. 44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate Cat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 00-4202 eBioscience ELISA Diluent Solution, 5X Cat. 00-4203 Super AquaBlue ELISA Substrate Cat. 14-7011 Affinity Purified anti-mouse IL-1a (Interleukin-1 alpha, IL1a) (clone ALF-161) |
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