Product Information

Contents: Biotin anti-human interleukin-10 (IL-10)
Catalog Number: 13-7109
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: JES3-12G8
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7109	Biotin anti-human IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA det ELISPOT det

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Description

The JES3-12G8 antibody reacts with human interleukin-10 (IL-10).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JES3-12G8 antibody has been reported for use in the detection of human IL-10 by ELISA.

Applications Tested

The JES3-12G8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-10 (IL-10) in combination with the purified JES3-9D7 (14-7108) antibody for capture and recombinant human IL-10 (14-8109) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

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