Catalog Number: 13-7099
Clone: 5D3-F7
Isotype: Mouse IgG1, κ
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|
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 17-7099 |
Allophycocyanin (APC) anti-human CCL2 (MCP-1, MCP1, MCAF) |
633 |
660 |
IC Flow
|
| 14-7099 |
Affinity Purified anti-human CCL2 (MCP-1, MCP1, MCAF) |
N/A |
N/A |
ELISA cap
IC Flow
IHC(Paraffin)
IP
|
| 16-7099 |
Functional Grade* Purified anti-human CCL2 (MCP-1, MCP1, MCAF) |
N/A |
N/A |
ELISA cap
NU
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 5D3-F7 antibody has been reported to be useful for detection of MCP-1 in a sandwich ELISA, neutralization of MCP-1 bioactivity, intracellular staining for flow cytometric analysis, IP, and immunohistochemical staining of frozen tissue sections.
Applications Tested
The biotinylated 5D3-F7 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the unlabelled 2H5 (
14-7096) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-6 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
The Functional Grade purified form of the 5D3-F7 antibody has been tested for neutralization of MCP-1 bioactivity. The fluorochrome-conjugated 5D3-F7 antibody has been tested for intracellular staining and flow cytometric analysis of human cells.
References
Peri, G., C. Milanese, C. Matteucci, C., L. Ruco, D. Zhou, S. Sozzani, I. Coletta, and A. Mantovani. 1994. A new monoclonal antibody (5D3-F7) which recognizes human monocyte-chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells. J. Immunol. Meth. 174: 249-257.
