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Catalog Number: 13-7091 Clone: 4E2 Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7091	Affinity Purified anti-mouse CCL2 (MCP-1, MCP1, MCAF)	N/A	N/A	ELISA cap

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Description

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The 4E2/MCP antibody reacts with mouse monocyte chemoattractant protein-1 (MCP-1).

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The 4E2/MCP antibody has been reported to be useful for detection and quantitation of MCP-1 in a sandwich ELISA.

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Applications Tested

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The biotinylated 4E2/MCP antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the unlabelled 2H5 (14-7096) antibody for detection and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.

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References

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Luo, Y., J. Laning, M. Hayashi, P.R. Hancock, B. Rollins, and M.E. Dorf. 1994. Serological analysis of the mouse beta-chemokine JE/mouse chemoattractant protein-1. J. Immunol. 153: 3708-3716.

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