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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe MQ2-39C3 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types. Applications ReportedFor research use only, not for diagnostic or therapeutic use. MQ2-39C3 has been reported for use in detection of human IL-6 by ELISA and immunoblotting (WB). Applications TestedThe MQ2-39C3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the purified MQ2-13A5 (14-7069) antibody for capture and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. References
Gaines Das, R. E. and S. Poole (1993). "The international standard for interleukin-6. Evaluation in an international collaborative study." J Immunol Methods 160(2): 147-53. Andersson, J., J. Abrams, et al. (1994). "Concomitant in vivo production of 19 different cytokines in human tonsils." Immunology 83(1): 16-24. Related ProductsCat. 00-0000 eBioscience 10X ELISA Coating Buffer Cat. 44-2404 Nunc MaxiSorp™ flat-bottom 96 well plate Cat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 00-4202 eBioscience ELISA Diluent Solution, 5X Cat. 00-4203 Super AquaBlue ELISA Substrate Cat. 14-7069 Affinity Purified anti-human IL-6 (Interleukin-6, IL6) (clone MQ2-13A5) Cat. 14-8069 Recombinant Human IL-6 (Interleukin-6, IL6) |
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