Product Information

Contents: Biotin anti-mouse interleukin-4 (IL-4)
Catalog Number: 13-7042
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: BVD6-24G2
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with PE anti-mouse CD4 (GK1.5) and intracellularly stained with FITC anti-mouse IL-4 (BVD6-24G2).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7042	FITC anti-mouse IL-4 (Interleukin-4, IL4)	488	518	IC Flow
13-7042	Biotin anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA det ELISPOT
14-7042	Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	IC Flow
25-7042	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IL-4 (Interleukin-4, IL4)	488	760	IC Flow

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Description

The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The BVD6-24G2 antibody is used for detection of mouse IL-4 by ELISA.

Applications Tested

The BVD6-24G2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-4 (IL-4) in combination with the purified 11B11 (14-7041) antibody for capture and recombinant mouse IL-4 (14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/ml - 4 pg/ml should be included in each ELISA plate.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.
Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.

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