Product Information

Contents: Biotin anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)
Catalog Number: 13-6644
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: DJR1
Isotype: Mouse IgG1, κ

Surface staining of human DR4 transfected cells with anti-human DR4 (DJR1) Biotin followed by SA-PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6644	Affinity Purified anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)	N/A	N/A	FC IH/F
13-6644	Biotin anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)	N/A	N/A	FC
12-6644	PE anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DJR1 monoclonal antibody reacts with human DR4, also known as TRAIL-R1. DR4 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR4 is expressed by a variety of human tumor cells and at very low level on human leukocytes.

Expression has also been found inside the cell on some cell lines (Zhang et al). This has been confirmed with intracellular staining of Jurkat cell line. Please contact tech@ebioscience.com for more information.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DJR1 antibody has been tested by flow cytometric analysis of human DR4-transfected cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ishiyama K, Ohdan H, Ohira M, Mitsuta H, Arihiro K, Asahara T. Difference in cytotoxicity against hepatocellular carcinoma between liver and periphery natural killer cells in humans. Hepatology. 2006 Feb;43(2):362-72. (DJR1, IHC frozen, PubMed)

Earel JK Jr, Vanoosten RL, Griffith TS. Histone deacetylase inhibitors modulate the sensitivity of tumor necrosis factor-related apoptosis-inducing ligand-resistant bladder tumor cells. Cancer Res. 2006 Jan 1;66(1):499-507. (DJR1, FC)

Zhang XD, Franco A, Myers K, Gray C, Nguyen T, Hersey P. Relation of TNF-related apoptosis-inducing ligand (TRAIL) receptor and FLICE-inhibitory protein expression to TRAIL-induced apoptosis of melanoma. Cancer Res. 1999 Jun 1;59(11):2747-53.

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