Product Information

Contents: Biotin anti-human DcR2 (CD264, TRAIL-R4, TRAILR4, TRUNDD, TNFRSF10D)
Catalog Number: 13-6239
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: DJR4-1
Isotype: Mouse IgG1, κ

Surface staining of human DcR2 transfected cells with anti-human DcR2 (DJR4-1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

The DJR4-1 monoclonal antibody reacts with human Decoy receptor 2 (DcR2), a transmembrane cell surface protein also known as TRAIL-R4 and TRUNDD. DcR2 has an extracellular TRAIL binding domain and a truncation in the death domain (DD) of its cytoplasmic tail. Along with DcR1, DcR2 binds to TRAIL and inhibits binding of TRAIL to DR4 or DR5 protecting cells from TRAIL-mediated cytotoxicity. DcR2 is expressed in many normal human tissues at low levels, but not in most tumors. Overexpression of DcR2 attenuates TRAIL-induced apoptosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR4-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DJR4-1 antibody has been tested by flow cytometric analysis of DcR2/293 transient transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gomez-Benito M, Martinez-Lorenzo MJ et al. 2007. Membrane expression of DR4, DR5 and caspase-8 levels, but not Mcl-1, determine sensitivity of human myeloma cells to Apo2L/TRAIL. Exp Cell Res. 2007 Jul 1;313(11):2378-88. (FC, PubMed)

Mulherkar N, Prasad KV et al. 2007. MADD/DENN splice variant of the IG20 gene is a negative regulator of caspase-8 activation. Knockdown enhances TRAIL-induced apoptosis of cancer cells. J Biol Chem. 2007 Apr 20;282(16):11715-21. (FC, PubMed)

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