Contents: Biotin anti-mouse IgA
Catalog Number: 13-5994
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.1% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 11-44-2
Isotype: Rat IgG1, κ
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|
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5994 |
Affinity Purified anti-mouse IgA |
N/A |
N/A |
FC
|
| 13-5994 |
Biotin anti-mouse IgA |
N/A |
N/A |
FC
|
| 12-5994 |
PE anti-mouse IgA |
488 |
575 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 11-44-2 monoclonal antibody reacts with the α heavy chain of mouse IgA. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgM. IgA is expressed as a membrane-bound form as well as a soluble dimer. Fluorochrome conjugated 11-44-2 is used as a detection secondary for mouse IgA.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 11-44-2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 11-44-2 antibody has been tested by flow cytometric analysis to detect a mouse IgA primary antibody. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Related Products
Cat. 13-4301 Biotin Rat IgG1 Isotype ControlCat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)Cat. 12-4317 Streptavidin-PE (Phycoerythrin)Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)
Mouse Cellular Antigens Chart