Product Information

Contents: Biotin anti-mouse Tie-2 (Tek, CD202)
Catalog Number: 13-5987
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: TEK4
Isotype: Rat IgG1, κ

Two-color surface staining of mouse bone marrow with anti-mouse CD202b (TEK4) PE and Sca-1 (D7) + c-Kit (2B8) APC. Total viable lineage negative cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5987	Affinity Purified anti-mouse Tie-2 (Tek, CD202)	N/A	N/A	FC IH/F
16-5987	Functional Grade* Purified anti-mouse Tie-2 (Tek, CD202)	N/A	N/A	FC
13-5987	Biotin anti-mouse Tie-2 (Tek, CD202)	N/A	N/A	FC
12-5987	PE anti-mouse Tie-2 (Tek, CD202)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit⁺, Sca1⁺ cells. Long-term multilineage repopulating cells were detected in Tie-2⁺, Lineage^-, c-Kit⁺, Sca1⁺ cells but not in Tie-2^-, Lineage^-, c-Kit⁺, Sca1⁺ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TEK4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This TEK4 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per 1 million cells in 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Tang Y, Borgstrom P, Maynard J, Koziol J, Hu Z, Garen A, Deisseroth A. Mapping of angiogenic markers for targeting of vectors to tumor vascular endothelial cells. Cancer Gene Ther. 2007 Apr;14(4):346-53. (TEK4, IHC frozen, PubMed)

Lelievre E, Bourbon PM, Duan LJ, Nussbaum RL, Fong GH. Deficiency in the p110alpha subunit of PI3K results in diminished Tie2 expression and Tie2(-/-)-like vascular defects in mice. Blood. 2005 May 15;105(10):3935-8. (TEK4, IHC, PubMed)

Kolatsi-Joannou M, Li XZ, Suda T, Yuan HT, Woolf AS. Expression and potential role of angiopoietins and Tie-2 in early development of the mouse metanephros. Dev Dyn. 2001 Sep;222(1):120-6.

Hsu HC, Ema H, Osawa M, Nakamura Y, Suda T, Nakauchi H. Hematopoietic stem cells express Tie-2 receptor in the murine fetal liver. Blood. 2000 Dec 1;96(12):3757-62.

Hamaguchi I, Huang XL, Takakura N, Tada J, Yamaguchi Y, Kodama H, Suda T. In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. Blood. 1999 Mar 1;93(5):1549-56.

Yano M, Iwama A, Nishio H, Suda J, Takada G, Suda T. Expression and function of murine receptor tyrosine kinases, TIE and TEK, in hematopoietic stem cells. Blood. 1997 Jun 15;89(12):4317-26.

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