mouse B7-DC antibody, Biotin conjugate

Contents: Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)
Catalog Number: 13-5986
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: TY25
Isotype: Rat IgG2a, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
13-5986-81	50 ug
13-5986-82	100 ug
13-5986-85	500 ug

Surface staining of B7-DC transfected cells with anti-mouse B7-DC (TY25) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5986	Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	N/A	N/A	FA FC IH/F IP WB
16-5986	Functional Grade* Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	N/A	N/A	FA FC
13-5986	Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	N/A	N/A	FC
12-5986	PE anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The TY25 monoclonal antibody reacts with mouse B7-DC/PD-L2. B7-DC, a member of the B7 family, has a predicted molecular weight of ~25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by sub-populations of dendritic cells, monocytes and macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, but binds PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. TY25 is a useful tool to study the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in mouse.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TY25 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The TY25 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and B7-DC transfected cells. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, Azuma M, Okumura K, Yagita H, Niimi M. 2004. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. Jan 15;77(1):6-12.
Tseng SY, Otsuji M, et al. 2001. B7-DC, a new dendritic cell molecule with potent costimulatory properties for T cells. J Exp Med 193(7): 839-46.
Carter L, Fouser L, et al. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol 32(3): 634-43.
Latchman Y, Wood CR, et al. 2001. PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol 2(3): 261-8.
Yamazaki T, Akiba H, et al. Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002 Nov 15;169(10):5538-45.
Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. 2003. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 171(8):4156-63.
Mohammed Javeed I. Ansari, Alan D. Salama, Tanuja Chitnis, R. Neal Smith, Hideo Yagita, Hisaya Akiba, Tomohide Yamazaki, Miyuki Azuma, Hideyuki Iwai, Samia J. Khoury, Hugh Auchincloss, Jr. and Mohamed H. Sayegh. 2003. The Programmed Death-1 (PD-1) Pathway Regulates Autoimmune Diabetes in Nonobese Diabetic (NOD) Mice. J Exp Med 198 (1): 63-69.

Tanaka K., M. Albin et al. 2007. PDL1 is required for peripheral transplantation tolerance and protection from chronic allograft rejection. J Immunol 179(8):5204-5210. (FA, PubMed)

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