Contents: Biotin anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)
Catalog Number: 13-5985
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: HK5.3
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Biotin mHK5.3 vs. Biotin Rat IgG2a Isotype Control (Mouse spleen cells.)
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5985 |
Affinity Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) |
N/A |
N/A |
FA
FC
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| 16-5985 |
Functional Grade* Purified anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) |
N/A |
N/A |
FA
FC
|
| 13-5985 |
Biotin anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) |
N/A |
N/A |
FC
|
| 12-5985 |
PE anti-mouse B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2) |
488 |
575 |
FC
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*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The HK5.3 monoclonal antibody reacts with mouse B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of ~40 kDa and belongs to the Ig superfamily. Mouse B7RP-1 is expressed by antigen presenting cells including B cells, monocytes/macrophages and dendritic cells. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HK5.3 antibody has been reported for use in flow cytometric analysis. HK5.3 is not recommended for use in immunohistochemical staining.
Applications Tested
The HK5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Iwai H, Kozono Y, et al. 2002. Amelioration of collagen-induced arthritis by blockade of inducible costimulator-b7 homologous protein costimulation. J Immunol. 169(8): 4332-9. Carreno BM, Collins M. 2002. THE B7 FAMILY OF LIGANDS AND ITS RECEPTORS: New Pathways for Costimulation and Inhibition of Immune Responses. Annu Rev Immunol. 20: 29-53. Liu X, Bai XF, et al. 2001. B7H costimulates clonal expansion of, and cognate destruction of tumor cells by, CD8(+) T lymphocytes in vivo. J Exp Med. 194(9): 1339-48.
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Mouse Cellular Antigens Chart