PLEASE NOTE: The MIH6 clone is being discontinued. We recommend clone MIH5, a better characterized sister clone, as an alternate product:
click here for more information
Contents: Biotin anti-mouse B7-H1 (PD-L1)
Catalog Number: 13-5984
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C. DO NOT FREEZE.
Clone: MIH6
Isotype: Rat IgG2a
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MIH6 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. Preliminary experiments suggest that MIH6 may not be a blocking antibody. For blocking in functional assays, clone MIH5 (catalog # 16-5982 ) is recommended.
Applications Reported
For research use only, not for diagnostic or therapeutic use. MIH6 has been reported for use in flow cytometric analysis.
References
Dong, H., G. Zhu, et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5(12): 1365-9.
Azuma, M. 2002. personal communication.
Freeman, G. J., A. J. Long, et al. 2000. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.
