mouse BTLA antibody, Biotin conjugate

Contents: Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking
Catalog Number: 13-5950
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 6F7
Isotype: Mouse IgG1, κ

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13-5950-80

25 ug

13-5950-82

100 ug

Staining of BALB/c splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.06 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (left) or 0.06 μg of Biotin anti-mouse BTLA (6F7) (right) followed by Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5950	Affinity Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking	N/A	N/A	FC
16-5950	Functional Grade* Purified anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking	N/A	N/A	FA FC
13-5950	Biotin anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking	N/A	N/A	FC
12-5950	Phycoerythrin (PE) anti-mouse BTLA (CD272, B and T lymphocyte attenuator) - Blocking	488	575	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA has been implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.

Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4⁺ and CD8⁺ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes . It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 6F7 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 6F7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Watanabe N, Gavrieli M, Sedy JR, Yang J, Fallarino F, Loftin SK, Hurchla MA, Zimmerman N, Sim J, Zang X, Murphy TL, Russell JH, Allison JP, Murphy KM. 2003. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 4 (7): 670-9.

Sedy JR, Gavrieli M, Potter KG, Hurchla MA, Lindsley RC, Hildner K, Scheu S, Pfeffer K, Ware CF, Murphy TL, Murphy KM. 2005. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. (6)1: 90-8.

Hurchla MA, Sedy JR, Gavrielli M, Drake CG, Murphy TL, Murphy KM. 2005. B and T Lymphocyte Attenuator exhibits structural expression polymorphisms and is highly induced in CD4+ T cells. The Journal of Immunology. 174: 3377-3385.

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