Product Information

Contents: Biotin anti-mouse CD95 Ligand (Fas Ligand, CD178)
Catalog Number: 13-5911
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MFL3
Isotype: Armenian Hamster IgG

Staining of mouse Fas Ligand-transfected L5178Y cells with 0.125 μg of Biotin Armenian Hamster IgG Iso Cntrl (cat. 13-4888) (open histogram) or 0.125 μg of Biotin anti-mouse FasL (MFL3) (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5911	PE anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	488	575	FC
13-5911	Biotin anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FC
14-5911	Affinity Purified anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
16-5911	Functional Grade* Purified anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MFL3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kayagaki, N., N. Yamaguchi, et al. (1997). "Polymorphism of murine Fas ligand that affects the biological activity." Proc Natl Acad Sci U S A 94(8): 3914-9.
Nakajima, A., H. Hirai, et al. (2000). "Treatment of lupus in NZB/W F1 mice with monoclonal antibody against fas ligand." J Autoimmun 14(2): 151-7.
Kayagaki, N., A. Kawasaki, et al. (1995). "Metalloproteinase-mediated release of human Fas ligand." J Exp Med 182(6): 1777-83.

Related Products

Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4888    Biotin Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 14-5912    Affinity Purified anti-mouse/rat CD95 Ligand (Fas Ligand, CD178, FasL) (clone MFL4)
Cat. 16-5912    Functional Grade Purified anti-mouse/rat CD95 Ligand (Fas Ligand, CD178, FasL) (clone MFL4)