Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Biotin
Also known as: FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
RUO: For Research Use Only
Staining of MC/9 cell line with 0.06 µg of Armenian Hamster IgG Isotype Control Biotin (cat.13-4888) (open histogram) or 0.06 µg of Anti-Mouse Fc epsilon Receptor I alpha (FcεR1) Biotin (filled histogram)followed by Streptavidin PE (cat.12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Biotin Catalog Number: 13-5898 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: MAR-1 Host/Isotype: Armenian Hamster IgG
Product Options
Cat. No.
Size
Price
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13-5898-81
50 ug
13-5898-82
100 ug
13-5898-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The MAR-1 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. FcεRIα forms a tetrameric complex with one β and two γ subunits. The β and γ subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MAR-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Arinobu Y, Iwasaki H, Gurish MF, Mizuno S, Shigematsu H, Ozawa H, Tenen DG, Austen KF, Akashi K. Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis.
Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18105-10. Epub 2005 Dec 5.(mar-1, FC, PubMed)
Obata K, Mukai K, Tsujimura Y, Ishiwata K, Kawano Y, Minegishi Y, Watanabe N, Karasuyama H. Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood. 2007 Aug 1;110(3):913-20. Epub 2007 Apr 4 (Mar-1, FC)
Sokol CL, Barton GM, Farr AG, Medzhitov R. A mechanism for the initiation of allergen-induced T helper type 2 responses.
Nat Immunol. 2008 Mar;9(3):310-8.(Mar-1, FA, PubMed)
Yamaguchi M., K. Hirai, A. Komiya, M. Miyamasu, Y. Furumoto, R. Teshima, K. Ohta, Y. Morita, S. J. Galli, C. Ra, K. Yamamoto. (2001) Regulation of Mouse Mast Cell Surface Fc epsilon RI expression by dexamethasone. Int Immunol 13(7):843-51.
