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Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Biotin

Also known as: FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor

RUO: For Research Use Only
mouse FceRI alpha antibody, Biotin conjugateStaining of MC/9 cell line with 0.06 µg of Armenian Hamster IgG Isotype Control Biotin (cat.13-4888) (open histogram) or 0.06 µg of Anti-Mouse Fc epsilon Receptor I alpha (FcεR1) Biotin (filled histogram)followed by Streptavidin PE (cat.12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Biotin
Catalog Number: 13-5898
Concentration: 0.5 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 2-8°C. Do not freeze.
Clone: MAR-1
Host/Isotype: Armenian Hamster IgG
 
Product Options
Cat. No. Size Price Add Qty to Cart
13-5898-81 50 ug
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13-5898-82 100 ug
13-5898-85 500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-4317 Streptavidin FITC
12-4317 Streptavidin PE
13-4888 Armenian Hamster IgG Isotype Control Biotin (eBio299Arm)
17-4317 Streptavidin APC
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-5898 Anti-Mouse Fc epsilon Receptor I alpha (FceR1) FITC 488 518 FC RUO
12-5898 Anti-Mouse Fc epsilon Receptor I alpha (FceR1) PE 488 575 FC RUO
13-5898 Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Biotin N/A N/A FC RUO
14-5898 Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Purified N/A N/A FA FC IHC (F) IP RUO
16-5898 Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Functional Grade* Purified N/A N/A FA FC RUO
51-5898 Anti-Mouse Fc epsilon Receptor I alpha (FceR1) Alexa Fluor® 647 633 668 FC RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The MAR-1 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. FcεRIα forms a tetrameric complex with one β and two γ subunits. The β and γ subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MAR-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Arinobu Y, Iwasaki H, Gurish MF, Mizuno S, Shigematsu H, Ozawa H, Tenen DG, Austen KF, Akashi K. Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18105-10. Epub 2005 Dec 5.(mar-1, FC, PubMed)

Obata K, Mukai K, Tsujimura Y, Ishiwata K, Kawano Y, Minegishi Y, Watanabe N, Karasuyama H. Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood. 2007 Aug 1;110(3):913-20. Epub 2007 Apr 4 (Mar-1, FC)

Sokol CL, Barton GM, Farr AG, Medzhitov R. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol. 2008 Mar;9(3):310-8.(Mar-1, FA, PubMed)

Yamaguchi M., K. Hirai, A. Komiya, M. Miyamasu, Y. Furumoto, R. Teshima, K. Ohta, Y. Morita, S. J. Galli, C. Ra, K. Yamamoto. (2001) Regulation of Mouse Mast Cell Surface Fc epsilon RI expression by dexamethasone. Int Immunol 13(7):843-51.

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