Product Information

Contents: Biotin anti-mouse KLRG1/MAFA
Catalog Number: 13-5893
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: 2F1
Isotype: Golden Syrian Hamster IgG

Staining of C57Bl/6 splenocytes with APC anti-NK1.1 (cat.17-5941) and 0.06µg of biotin Golden Syrian hamster IgG isotype control (cat.13-4913) (left) or 0.06 µg of biotin 2F1 (right) followed by SAv-PE (cat.12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5893	Affinity Purified anti-mouse KLRG1/MAFA	N/A	N/A	FC IP
16-5893	Functional Grade* Purified anti-mouse KLRG1/MAFA	N/A	N/A	FA FC
13-5893	Biotin anti-mouse KLRG1/MAFA	N/A	N/A	FC
11-5893	Fluorescein isothiocyanate (FITC) anti-mouse KLRG1/MAFA	488	518	FC
17-5893	Allophycocyanin (APC) anti-mouse KLRG1/MAFA	633	660	FC
25-5893	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse KLRG1/MAFA	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported

For research use only, not for diagnostic or therapeutic use. 2F1 has been reported for use in flow cytometric analysis.

Applications Tested

The 2F1 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions and can be used at less than or equal to 0.06 µg per million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Beyersdorf NB, Ding X, Karp K, Hanke T. 2001. Expression of inhibitory "killer cell lectin-like receptor G1" identifies unique subpopulations of effector and memory CD8 T cells. Eur J Immunol. 31:3443-52

Voehringer D, Blaser C, Brawand P, Raulet DH, Hanke T, Pircher H. 2001. Viral infections induce abundant numbers of senescent CD8 T cells. J Immunol. 167:4838-43

Corral L, Hanke T, Vance RE, Cado D, Raulet DH. 2000. NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules. Eur J Immunol. 30:920-30

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4914  Biotin Golden Syrian Hamster IgG Isotype Control (clone n/a)