Product Information

Contents: Biotin anti-mouse IgM
Catalog Number: 13-5890
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.1% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eB121-15F9
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.25 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.25 μg of Biotin eB121-15F9 (right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5890	Affinity Purified anti-mouse IgM	N/A	N/A	FC
13-5890	Biotin anti-mouse IgM	N/A	N/A	FC
11-5890	FITC anti-mouse IgM	488	518	FC
12-5890	PE anti-mouse IgM	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Applications Reported

For research use only, not for diagnostic or therapeutic use. eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. It can also be used in ELISA.

Applications Tested

The eB121-15F9 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control
Cat. 11-5790  Fluorescein (FITC) anti-mouse IgM (clone II/41)
Cat. 12-5790  Phycoerythrin (PE) anti-mouse IgM (clone II/41)
Cat. 13-5790  Biotin anti-mouse IgM (clone II/41)
Cat. 14-5790  Affinity Purified anti-mouse IgM (clone II/41)
Cat. 17-5790  Allophycocyanin (APC) anti-mouse IgM (clone II/41)