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Mouse Cellular Antigens Chart

Contents: Biotin anti-mouse IgM Catalog Number: 13-5890 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.1% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: eB121-15F9 Isotype: Rat IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 13-5890-81 50 ug $75 13-5890-82 100 ug $100 13-5890-85 500 ug $185 *International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.25 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.25 μg of Biotin eB121-15F9 (right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5890	FITC anti-mouse IgM	488	518	FC
12-5890	PE anti-mouse IgM	488	575	FC
14-5890	Affinity Purified anti-mouse IgM	N/A	N/A	FC
13-5890	Biotin anti-mouse IgM	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs. It can also be used in ELISA.

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Applications Tested

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The eB121-15F9 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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Related Products

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Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321    Biotin Rat IgG2a Isotype Control
Cat. 11-5790    Fluorescein (FITC) anti-mouse IgM (clone II/41)
Cat. 12-5790    Phycoerythrin (PE) anti-mouse IgM (clone II/41)
Cat. 13-5790    Biotin anti-mouse IgM (clone II/41)
Cat. 14-5790    Affinity Purified anti-mouse IgM (clone II/41)
Cat. 17-5790    Allophycocyanin (APC) anti-mouse IgM (clone II/41)