Contents: Biotin anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2)
Catalog Number: 13-5888
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: MIH18
Isotype: Mouse IgG1, κ
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Staining of in vitro derived human dendritic cells with APC anti-human CD209/DC-SIGN (eB-h209) (cat. 17-2099) and 0.125 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4719) (left) or 0.125 μg of Biotin MIH18 (right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-5888 |
Affinity Purified anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
N/A |
N/A |
FC
|
| 16-5888 |
Functional Grade* Purified anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
N/A |
N/A |
FA
FC
|
| 13-5888 |
Biotin anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
N/A |
N/A |
FC
|
| 12-5888 |
PE anti-human B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
488 |
575 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The MIH18 monoclonal antibody reacts with human B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. MIH18 is a useful tool for studies to elucidate the exact function of B7-DC/PD-L2 in APC/T cell interaction and to characterize the expression pattern of this molecule in humans. MIH18 is a blocking antibody.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MIH18 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The MIH18 antibody has been tested by flow cytometric analysis of mature dendritic cells and human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Pfistershammer K, Klauser C, Pickl WF, Stockl J, Leitner J, Zlabinger G, Majdic O, Steinberger P.No evidence for dualism in function and receptors: PD-L2/B7-DC is an inhibitory regulator of human T cell activation.
Eur J Immunol. 2006 May;36(5):1104-13.(MIH18, FA
PubMed)
Youngnak-Piboonratanakit P, Tsushima F, Otsuki N, Igarashi H, Machida U, Iwai H, Takahashi Y, Omura K, Yokozeki H, Azuma M. 2004. The expression of B7-H1 on keratinocytes in chronic inflammatory mucocutaneous disease and its regulatory role. Immunol Lett. 15;94(3):215-22. (MIH18, IHC frozen)
Related Products
Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate)Cat. 12-4317 Streptavidin-PE (Phycoerythrin)Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC)Cat. 13-4714 Biotin Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart