Product Information

Contents: Biotin anti-mouse NKG2D / CD314 (blocking)
Catalog Number: 13-5882
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: CX5
Isotype: Rat IgG1, κ

Staining of C57Bl/6 splenocytes with APC anti-mouse NK1.1 (cat.17-5941) and 0.06 µg of biotin rat IgG1 isotype control (cat.13-4301) (left) or 0.06 µg of biotin CX5 (right) followed by SAv-PE (cat.12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5882	Affinity Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
16-5882	Functional Grade* Purified anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FA FC
13-5882	Biotin anti-mouse NKG2D / CD314 (blocking)	N/A	N/A	FC
12-5882	PE anti-mouse NKG2D / CD314 (blocking)	488	575	FC
17-5882	APC anti-mouse NKG2D / CD314 (blocking)	633	660	FC
25-5882	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NKG2D / CD314 (blocking)	488	760	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1α, -β, -γ, -δ, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1⁺ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CX5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lodoen M, Ogasawara K, Hamerman JA, Arase H, Houchins JP, Mocarski ES, Lanier LL. 2003. NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules. J Exp Med. 197(10):1245-53.
Cerwenka A, Baron JL, Lanier LL. 2001. Ectopic expression of retinoic acid early inducible-1 gene (RAE-1) permits natural killer cell-mediated rejection of a MHC class I-bearing tumor in vivo. Proc Natl Acad Sci U S A. 98(20):11521-6.
Cerwenka A, Bakker AB, McClanahan T, Wagner J, Wu J, Phillips JH, Lanier LL. 2000. Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice. Immunity. 12(6):721-7.

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