Staining of Yac-1 cell line with 0.125 µg of Rat IgG2b κ Isotype Control Biotin (cat. 13-4031) (open histogram) or 0.125 µg of Anti-Mouse RAE1λ Biotin (filled histogram) followed by Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse RAE1 gamma Biotin Catalog Number: 13-5881 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: CX1 Host/Isotype: Rat IgG2b, κ
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Cat. No.
Size
Price
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13-5881-81
50 ug
13-5881-82
100 ug
13-5881-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The CX1 monoclonal antibody reacts with the mouse retinoic acid early inducible gamma (RAE-1γ) and weakly cross-reacts with the RAE-1α and β, but not with δ and ε. These 5 members of the RAE-1 family, along with another protein encoded by the minor histocompatibility gene complex called H60 have been identified as ligands for the mouse NKG2D molecule. These ligands are normally absent from adult tissue, however the presence of retinoic acid or certain disease conditions (such as tumors) can induce up-regulation of this protein at the cell surface. RAE-1α, -β, and -γ and H60 are expressed in BALB/c mice, but not in C57BL/6, whereas RAE-1δ, and -ε are expressed in C57BL/6 mice. It is reported that in NOD mice, both NKG2D and its ligand RAE-1 are expressed on the same NK cells, resulting in self-modulation of NKG2D expression and function.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The CX1 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The CX1 antibody has been tested by flow cytometric analysis of YAC-1 cell line and mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Cerwenka, A., A.B. Bakker, T. McClanahan, J. Wagner, J. Wu, J.H. Phillips, L.L. Lanier. (2000). “Retinoic acid early inducible genes define a ligand family for the activating NKG2D receptor in mice.” Immunity. 12(6): 721-27. Diefenbach, A., E.R. Jensen, A.M. Jamieson, D.H. Raulet. (2001). “Rae-1 and H60 ligands of the NKG2D receptor stimulate tumour immunity.” Nature. 413(6852): 165-71. Lodoen, M., K. Ogasawara, J.A. Hamerman, H. Arase, J.P. Houchins, E.S. Mocarski, L.L. Lanier. (2003). “NKG2D-mediated natural killer cell protection against cytomegalovirus is impaired by viral gp40 modulation of retinoic acid early inducible 1 gene molecules.” 197(10): 1245-53.
