Biotin anti-mouse GITR antibody: anti-TNFRSF18 antibody: biotin conjugate

Contents: Biotin anti-mouse GITR (TNFRSF18)
Catalog Number: 13-5874
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: DTA-1
Isotype: Rat IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
13-5874-80	25 ug	$105
13-5874-82	100 ug	$275

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with APC anti-mouse CD25 (PC61.5) (cat. 17-0251) and 0.06 μg of PE Rat IgG2b Iso Cntrl (cat. 12-4031) (left) or 0.0002 μg of PE DTA-1 (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5874	Fluorescein isothiocyanate (FITC) anti-mouse GITR (TNFRSF18, AITR)	488	518	FC
12-5874	Phycoerythrin (PE) anti-mouse GITR (TNFRSF18, AITR)	488	575	FC
13-5874	Biotin anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FC
14-5874	Affinity Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC IP
16-5874	Functional Grade* Purified anti-mouse GITR (TNFRSF18, AITR)	N/A	N/A	FA FC
17-5874	Allophycocyanin (APC) anti-mouse GITR (TNFRSF18, AITR)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naïve mice, GITR is expressed predominantly by CD4⁺CD25⁺ T regulatory cells (Treg) and by CD25⁺ CD4⁺ CD8^- thymocytes. Stimulation of GITR abrogates Treg cell-mediated suppression. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DTA-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ng (0.0005 μg) per million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Note: The concentration suggested above has been modified from previous lots of biotin-conjugated DTA-1 antibody.

References

Shimizu J, Yamazaki S, Takahashi T, Ishida Y, Sakaguchi S. Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance. Nat Immunol. 2002 Feb;3(2):135-42.

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