Staining of normal human peripheral blood cells with Anti-Human CD8 APC (cat. 17-0086) and 0.125 µg of Mouse IgG1 κ Isotype Control Biotin (cat. 13-4714) (left) or 0.125 µg of Anti-Human CD244 Biotin (right) followed by Streptavidin PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD244 Biotin Catalog Number: 13-5838 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: eBioC1.7 (C1.7) Host/Isotype: Mouse IgG1, κ
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Size
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13-5838-81
50 ug
13-5838-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, γδ T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. For CD244 expressed on NK cells, binding of CD48 results in enhanced NK cell cytotoxicity and secretion of IFN-γ. Recently, it was demonstrated that binding of the C1.7 monoclonal antibody and CD48 involve the same residue in the V domain of human CD244, which explains the ability of C1.7 binding to induce activation of NK cells. Binding of C1.7 to CD244 leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP which renders it unable to bind to phosphorylated CD244.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioC1.7 (C1.7) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioC1.7 (C1.7) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Valiante NM, Trinchieri G.
Identification of a novel signal transduction surface molecule on human cytotoxic lymphocytes.
J Exp Med. 1993 Oct 1;178(4):1397-406. (C1.7, mAb development, FC, FA, WB, PubMed)
Chuang SS, Kim MH, Johnson LA, Albertsson P, Kitson RP, Nannmark U, Goldfarb RH, Mathew PA.
2B4 stimulation of YT cells induces natural killer cell cytolytic function and invasiveness.
Immunology. 2000 Jul;100(3):378-83. (C1.7, FC, FA, PubMed)
Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA.
Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells.
J Immunol. 2005 Jul 15;175(2):1005-13. (C1.7, FC, PubMed)
van Emmerik NE, Knoop CJ, Vaessen LM, Balk AH, Mochtar B, Claas FH, Weimar W.
C1.7 monoclonal antibody designates high-avidity CD4+ cytotoxic T lymphocytes involved in clinical heart rejection.
Transplantation. 1998 Jul 15;66(1):135-8. (C1.7, FC, PubMed)
