Product Information

Contents: Biotin anti-mouse V alpha 8 TCR (Vα8-TCR, Vα-8 TCR)
Catalog Number: 13-5819
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: CTVA8
Isotype: Rat IgG2a

Staining of C57Bl/6 lymph node cells with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.25 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.25 μg of Biotin anti-mouse Va-8 TCR (CTVA8) (right) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-5819	Biotin anti-mouse V alpha 8 TCR (Va8-TCR, Va-8 TCR)	N/A	N/A	FC
11-5819	Fluorescein isothiocyanate (FITC) anti-mouse V alpha 8 TCR (Va8-TCR, Va-8 TCR)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CTVA8 monoclonal antibody reacts with T cell receptors (TCR) which contain Vα 8, which is expressed by most known mouse strains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CTVA8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This CTVA8 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control