Product Information

Contents: Biotin anti-mouse γδTCR
Catalog Number: 13-5811
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: UC7-13D5
Isotype: Armenian Hamster IgG

Mouse (C57BL/6) lymph node cells were stained with biotinylated UC7-13D5 and FITC anti-mouse CD3 (145-2C11, cat.no. 11-0031). The binding of biotinylated UC7-13D5 was detected with Streptavidin-PE (cat.no. 12-4317)

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5811	FITC anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	488	518	FC
13-5811	Biotin anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	N/A	N/A	FC
14-5811	Affinity Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	N/A	N/A	FC IP
16-5811	Functional Grade* Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR, TCRgd)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The UC7-13D5 monoclonal antibody reacts with the mouse γδ TCR complex. γδ TCR is expressed by a small subset of T cells in the thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. The exact specificity, ligand and function of γδ TCR-bearing T cells are not yet fully understood; it is suggested that these cells recognize bacterial ligands and some tumor cells in the context of MHC class I-like gene products and play a role in regulation of the immune response and during bacterial infection.

Applications Reported

For research use only, not for diagnostic or therapeutic use. UC7-13D5 has been reported for use as a phenotypic marker for γδ TCR-expressing T cells in flow cytometric analysis.

Applications Tested

The UC7-13D5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Taguchi, T., W. K. Aicher, et al. (1991). "Novel function for intestinal intraepithelial lymphocytes. Murine CD3+, gamma/delta TCR+ T cells produce IFN-gamma and IL-5." J Immunol 147(11): 3736-44.
Kakkanaiah, V. N., M. Nagarkatti, et al. (1991). "CD4-CD8- thymocytes from MRL-lpr/lpr mice exhibit abnormal proportions of alpha beta- and gamma delta-TCR+ cells and demonstrate defective responsiveness when activated through the TCR." Cell Immunol 137(2): 269-82.
Bluestone, J. A., R. Q. Cron, et al. (1991). "Repertoire development and ligand specificity of murine TCR gamma delta cells." Immunol Rev 120: 5-33.

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Cat. 13-4888    Biotin Armenian Hamster IgG Isotype Control (clone eBio299Arm)