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Mouse Cellular Antigens Chart

Contents: Biotin anti-mouse IgM Catalog Number: 13-5790 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. Clone: II/41 Isotype: Rat IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 13-5790-81 50 ug $75 13-5790-82 100 ug $100 13-5790-85 500 ug $185 *International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 bone marrow cells with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.06 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.06 μg of Biotin II/41 (right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5790	Fluorescein (FITC) anti-mouse IgM	488	518	FC
12-5790	Phycoerythrin (PE) anti-mouse IgM	488	575	FC
15-5790	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse IgM	488	670	FC
17-5790	Allophycocyanin (APC) anti-mouse IgM	633	660	FC
25-5790	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IgM	488	760	FC
35-5790	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse IgM	488	690	FC
36-5790	Functional Grade* Biotin anti-mouse IgM	N/A	N/A	FC
14-5790	Affinity Purified anti-mouse IgM	N/A	N/A	ELISA  FC  IH/F
13-5790	Biotin anti-mouse IgM	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The II/41 monoclonal antibody reacts with the μ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The II/41 antibody has been reported for use in flow cytometric analysis.

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Applications Tested

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The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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References

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Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. 1993. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 150(12):5252-62.

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Related Products

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Cat. 11-4317    Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317    Streptavidin-PE (Phycoerythrin)
Cat. 17-4317    Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321    Biotin Rat IgG2a Isotype Control
Cat. 11-5890    FITC anti-mouse IgM (clone eB121-15F9)
Cat. 12-5890    PE anti-mouse IgM (clone eB121-15F9)
Cat. 13-5890    Biotin anti-mouse IgM (clone eB121-15F9)
Cat. 14-5890    Affinity Purified anti-mouse IgM (clone eB121-15F9)