Product Information

Contents: Biotin anti-human Nanog
Catalog Number: 13-5769
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: hNanog.1
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5769	Affinity Purified Mouse anti-human Nanog monoclonal	N/A	N/A	IHC(Paraffin) WB
13-5769	Biotin Mouse anti-human Nanog	N/A	N/A	IC Flow
11-5769	Fluorescein isothiocyanate (FITC) Mouse anti-human Nanog	488	518	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The hNanog.1 antibody was generated against and reacts with human Nanog, a homeobox transcription factor. In addition to the known Oct4 and Stat3 transcriptional operating systems, Nanog plays a crucial role in the second embryonic cell fate specification following formation of the blastocyst. Nanog was identified as a gene specifically expressed in mouse ES cells and preimplantation embryos and is capable of maintaining ES cell self-renewal independently of LIF/Stat3. Potential cross-reactivity of this antibody to other proteins has not been determined.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This hNanog.1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This hNanog.1 antibody has been tested by flow cytometric analysis of NTERA embryonic stem cells. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K, Maruyama M, Maeda M, Yamanaka S. 2003. The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 113(5):631-42.

Cavaleri F, Scholer HR. 2003. Nanog: a new recruit to the embryonic stem cell orchestra. Cell. 113(5):551-2

Hoei-Hansen CE, Kraggerud SM, et al. 2007. Ovarian dysgerminomas are characterised by frequent KIT mutations and abundant expression of pluripotency markers. Mol Cancer 2;6:12. (hNanog.1, IHC paraffin, PubMed)

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