Product Information

Contents: Biotin anti-mouse gamma delta TCR (γδ-TCR, γδTCR)
Catalog Number: 13-5711
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioGL3 (GL-3, GL3)
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5711	Affinity Purified anti-mouse gamma delta TCR (gd-TCR, gdTCR)	N/A	N/A	FC IP
13-5711	Biotin anti-mouse gamma delta TCR (gd-TCR, gdTCR)	N/A	N/A	FC
11-5711	Fluorescein isothiocyanate (FITC) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	488	518	FC
12-5711	Phycoerythrin (PE) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	488	575	FC
15-5711	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	488	670	FC
17-5711	Allophycocyanin (APC) anti-mouse gamma delta TCR (gd-TCR, gdTCR)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The GL3 monoclonal antibody (mAb) reacts with the γδ T-cell Receptor complex (TCR) on all γδ TCR bearing T cells, but it does not react with aß TCR. The γδ TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.

It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.

Applications Tested

This GL3 (GL-3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Goodman, T., and L. LeFrançois. 1989. Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J. Exp. Med. 170: 1569-1581.

MacDonald, H. R., M. Schreyer, R. C. Howe, and C. Bron. 1990. Selective expression of CD8a (Ly-2) subunit on activated thymic γδ cells. Eur. J. Immunol. 20: 927-930.

LeFrançois, L. 1991. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J. Immunol. 147: 1746-1751.

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