Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The GL3 monoclonal antibody (mAb) reacts with the γδ T-cell Receptor
complex (TCR) on all γδ TCR bearing T cells, but it does not react with aß
TCR. The γδ TCR is present on cells in the thymus, epidermis, epithelial lining
of the intestine, peritoneal cavity, and lymphoid tissues.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This GL3 (GL-3) antibody has been reported for use in flow cytometric analysis.
It is recommended to prestain cells with anti-mouse CD16/32 (cat. 14-0161) to prevent non-specific Fc-mediated binding of the GL3 monoclonal antibody.
Applications Tested
This GL3 (GL-3) antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Goodman, T., and L. LeFrançois. 1989. Intraepithelial lymphocytes.
Anatomical site, not T cell receptor form, dictates phenotype and
function. J. Exp. Med. 170: 1569-1581.
MacDonald, H. R., M. Schreyer, R. C. Howe, and C. Bron. 1990.
Selective expression of CD8a (Ly-2) subunit on activated thymic γδ
cells. Eur. J. Immunol. 20: 927-930.
LeFrançois, L. 1991. Phenotypic complexity of intraepithelial
lymphocytes of the small intestine. J. Immunol. 147: 1746-1751.
