Staining of NIH/3T3 cells with 0.25 µg of Rat IgG1 κ Isotype Control Biotin (cat. 13-4301) (open histogram) or 0.25 µg of Anti-Mouse Lymphotoxinβ Receptor Biotin (filled histogram) followed by Streptavidin PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Anti-Mouse Lymphotoxin beta Receptor Biotin Catalog Number: 13-5671 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: eBio3C8 (3C8) Host/Isotype: Rat IgG1, κ
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Size
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13-5671-80
25 ug
13-5671-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The antibody reacts with mouse lymphotoxin-beta receptor (LTBR). Northern blot analysis of tissues from adult mice showed that expression levels of LTBR mRNA were strong in lung, liver, and kidney, moderate in heart and testes, but weak in brain, thymus, spleen, and lymph nodes. The tumor necrosis factor receptor-related protein is the human receptor for the heterotrimer of lymphotoxin-alpha and lymphotoxin-beta. This LT-alpha/LT-beta heterotrimer (LT-α1β2) is assumed to take part in immunologic reactions by cell-cell contact, but does not bind to either TNFR1 (CD120α) or TNFR2 (CD120β). LTBR is expressed by day 7 embryos, so it has been speculated that the LT-alpha/LT-beta receptor system may also have some function in early embryogenesis.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio3C8 (3C8) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio3C8 (3C8) antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended to block Fcγ-receptors with anti-mouse CD16/32 (cat. 14-0161), prior to staining to prevent non-specific binding of eBio3C8. Furthermore, it is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Basak S, Kim H, Kearns JD, Tergaonkar V, O'Dea E, Werner SL, Benedict CA, Ware CF, Ghosh G, Verma IM, Hoffmann A. A fourth IkappaB protein within the NF-kappaB signaling module.
Cell. 2007 Jan 26;128(2):369-81. (3C8, FA, PubMed)
Chin RK, Lo JC, Kim O, Blink SE, Christiansen PA, Peterson P, Wang Y, Ware C, Fu YX. Lymphotoxin pathway directs thymic Aire expression.
Nat Immunol. 2003 Nov;4(11):1121-7. Epub 2003 Sep 28.(3C8, FA, PubMed)
Nakamura, T., et al. 1995. The murine lymphotoxin-beta receptor cDNA: isolation by the signal sequence trap and chromosomal mapping. Genomics 30: 312-319.
Crowe, P., et al. 1996. A lymphotoxin-beta-specific receptor. Science 264: 707-710.
Dejardin E, Droin NM, Delhase M, Haas E, Cao Y, Makris C, Li ZW, Karin M, Ware CF, Green DR.
The lymphotoxin-beta receptor induces different patterns of gene expression via two NF-kappaB pathways.
Immunity. 2002 Oct;17(4):525-35.
