Intracellular staining of the monocyte population in human PBMCs (right) or C57 mouse bone marrow cells (left) with APC anti-mouse CD11b (M1/70) (cat. 17-0112) and 0.125 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (left) or 0.125 μg of Biotin anti-mouse Galectin-3 (M3/38) (right) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Biotin anti-mouse Galectin-3 / Mac-2 Catalog Number: 13-5301 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: eBioM3/38 (M3/38) Host/Isotype: Rat IgG2a, κ Storage Conditions: Store at 4°C. DO NOT FREEZE.
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Size
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13-5301-81
50 ug
13-5301-82
100 ug
13-5301-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.
This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioM3/38 (M3/38) antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Staining can be done using Foxp3 buffers (cat. 00-5523) or IC Fixation Buffers (cat. 88-8823)
References
Ho MK, Springer TA. 1982. MAC-2, A Novel 32000 Mr Mouse Macrophage Subpopulation-Specific Antigen Defined by Monoclonal Antibodies. J. Immunol. 128(3):1221-1228.
Joo G-H, Goedegebuure PS, Sadanaga N, Nagoshi M, von Bernstorff W, Eberlein TJ. Expression and function of galectin-3, a b-galactoside-binding protein in activated T lymphocytes J. Leukoc. Biol. 69: 555–564; 2001.
Oliveira FL, Fraza P, Chammas R, Hsu DK, Liu FT, Borojevic R, Takiya CM,
El-Cheikh MC, Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice J. Leukoc. Biol. 82: ePub; 2007
Springer TA, 1981. Monoclonal Antibody Analysis of Complex Biological Systems. J. Biol Chem. 256(8):3833-3839.
