Product Information

Contents: Biotin goat anti-human IgG Fcg Fragment Specific, Minimal Reactivity to Mouse IgG
Catalog Number: 13-4998
Sizes: 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-4998	Phycoerythrin (PE) Anti-Human IgG Fcg Fragment Specific, Minimal Reactivity to Mouse IgG	488	575	FC
13-4998	Biotin Anti-Human IgG Fcg Fragment Specific, Minimal Reactivity to Mouse IgG	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This goat antibody reacts with the Fc fragment of heavy chains on human IgG but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM or IgA, or against nonimmunoglobulin serum proteins. This antibody has been tested to ensure minimal cross-reaction with bovine, horse, and mouse serum proteins, but the antibody may cross-react with immunoglobulins from other species.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Biotin goat anti-Human IgG (Fcg Frag Specific) has been reported for use in flow cytometric analysis.

Applications Tested

Biotin goat anti-Human IgG (Fcg Frag Specific) has been tested by flow cytometric analysis to detect purified human Fc-tagged proteins. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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