Product Information

Contents: Biotin mouse anti-rat IgG (H+L)
Catalog Number: 13-4813
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.75% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Mouse IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-4813	Biotin Anti-Rat IgG	N/A	N/A	ELISA FC IH/F WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This antibody will recognize the light chains of other rat immunoglobulins. Reactions to non-immunoglobulin rat serum proteins have not been detected.

Applications Reported

For research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified rat immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometric analysis.

Applications Tested

Biotin Anti-Rat IgG has been tested by flow cytometric analysis to detect purified rat monoclonal antibodies. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse and mouse serum proteins, but may cross-react with immunoglobulins from other species.

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