Contents: Biotin mouse anti-rat IgG (H+L)
Catalog Number: 13-4813
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.75% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Mouse IgG
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 13-4813 |
Biotin Anti-Rat IgG |
N/A |
N/A |
ELISA
FC
IH/F
WB
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Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
This antibody will recognize the light chains of other rat immunoglobulins. Reactions to non-immunoglobulin rat serum proteins have not been detected.
Applications Reported
For research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified rat immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometric analysis.
Applications Tested
Biotin Anti-Rat IgG has been tested by flow cytometric analysis to detect purified rat monoclonal antibodies. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse and mouse serum proteins, but may cross-react with immunoglobulins from other species.
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