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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThis antibody will recognize the light chains of other rat immunoglobulins. Reactions to non-immunoglobulin rat serum proteins have not been detected. Applications ReportedFor research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified rat immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometric analysis. Applications TestedBiotin Anti-Rat IgG has been tested by flow cytometric analysis to detect purified rat monoclonal antibodies. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse and mouse serum proteins, but may cross-react with immunoglobulins from other species. Related ProductsCat. 18-4100 Avidin Horse Radish Peroxidase (AV-HRP) Cat. 11-4317 Streptavidin-FITC (Fluorescein isothiocyanate) Cat. 12-4317 Streptavidin-PE (Phycoerythrin) Cat. 17-4317 Streptavidin Allophycocyanin (SA-APC) |
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Copyright © 2000-2008 eBioscience, Inc. Product For Research Use Only: Not for further distribution without written consent. |