Product Information

Contents: Biotin goat anti-Syrian hamster IgG (H+L)
Catalog Number: 13-4213
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.6, 250 mM NaCl, 0.09% NaN₃, 1.5% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Goat IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-4213	Biotin Anti-Syrian Hamster IgG	N/A	N/A	ELISA FC IHC WB
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This antibody reacts with the heavy chains on Syrian hamster IgG and with light chains common to most Syrian hamster immunoglobulins. Reactions to non-immunoglobulin hamster proteins have not been detected.

Applications Reported

For research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified Syrian hamster immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometry. At high concentrations, cross-reaction to Armenian hamster IgG has been observed.

Applications Tested

Biotin Anti-Syrian Hamster IgG has been tested by flow cytometric analysis to detect purified Syrian hamster monoclonal antibodies. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, rabbit, rat, and mouse serum proteins, but may cross-react with immunoglobulins from other species.

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