Product Information

Contents: Biotin anti-Phycoerythrin (anti-PE)
Catalog Number: 13-4120
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioPE-DLF
Isotype: Mouse IgG1, κ

Staining of C57Bl/6 splenocytes with PE anti-mo/hu B220 (RA3-6B2) (cat. 12-0452) and 0.06 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (left) or 0.06 μg of Biotin anti-PE (right) followed by Streptavidin-APC (cat. 17-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4120	Purified anti-Phycoerythrin (anti-PE)	N/A	N/A	FC
13-4120	Biotin anti-Phycoerythrin (anti-PE)	N/A	N/A	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioPE-DLF antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Guy K, Crichton DN, Ross JA. Indirect immunofluorescence labelling with complexes of phycoerythrin and monoclonal anti-phycoerythrin antibodies (PEAPE complexes). J Immunol Methods. 1988 Sep 13;112(2):261-5.

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714  Biotin Mouse IgG1, K Isotype Control