Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
This antibody reacts with the heavy chains on Armenian hamster IgG and with the light chains common to most Armenian hamster immunoglobulins. Reactions to non-immunoglobulin hamster proteins have not been detected.
Applications Reported
For research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV), this polyclonal antibody can be used for detection of purified Armenian hamster immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometry. At high concentrations, cross-reaction to Syrian hamster IgG has been observed.
Applications Tested
Biotin Anti-Armenian Hamster IgG has been tested by flow cytometric analysis to detect purified Armenian hamster monoclonal antibodies. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, rabbit, rat, and mouse serum proteins, but may cross-react with immunoglobulins from other species.
