Contents: Biotin rat anti-mouse IgG (H+L)
Catalog Number: 13-4013
Formulation: Phosphate buffer pH 7.6,
250 mM NaCl, 0.05% NaN3,
1.5% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: Polyclonal
Isotype: Rat IgG
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 13-4013 |
Biotin Anti-Mouse IgG |
N/A |
N/A |
ELISA
FC
IHC
WB
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Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The anti-mouse IgG rat polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reaction to human, bovine, horse, rat and rabbit serum proteins. Reactions to non-immunoglobulin mouse serum proteins have not been detected. This antibody will recognize the light chains of other mouse immunoglobulins.
Applications Reported
For research use only, not for diagnostic or therapeutic use. In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified mouse immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometry.
Applications Tested
This polyclonal antibody has been tested by flow cytometric analysis to detect purified mouse monoclonal antibodies. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse and mouse serum proteins, but may cross-react with immunoglobulins from other species.
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