Staining of SJL splenocytes with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.06 μg of Biotin Rat IgG2b Iso Cntrl (cat. 13-4331) (left) or 0.06 μg Biotin Mouse PDCA-1 (pDC Ag-1, BST2, CD317) (right) followed by Streptavidin-APC (cat. 17-4317). Cells in the large forward scatter population were used for analysis.
Contents: Biotin anti-mouse PDCA-1 (pDC Ag-1, BST2, CD317) Catalog Number: 13-3172 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: eBio927 Host/Isotype: Rat IgG2b Storage Conditions: Store at 4°C. DO NOT FREEZE.
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Size
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13-3172-81
50 ug
13-3172-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.
The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio927 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio927 antibody has been tested by flow cytometric analysis of SJL splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Blasius A.L., E. Giurisat, M. Cella, R.D. Schreiber, A.S. Shaw, M. Colonna. 2006. Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naïve Mouse, but a Promiscuous Cell Surface Antigen Following IFN Stimulation. J Immunol. 177:3260-3265 (927, FC, FA)
