Product Information

Contents: Biotin anti-mouse CIRE (DC-SIGN, CD209)
Catalog Number: 13-2092
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: LWC06
Isotype: Rat IgG2a

Staining of mouse CIRE/DC-SIGN-transfected CHO cells with 0.5 μg of Biotin Rat IgG2a Iso Cntrl (cat. 13-4321) (open histogram) or 0.5 μg of Biotin anti-mouse CIRE (LWC06) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-2092	Affinity Purified anti-mouse CIRE (DC-SIGN, CD209)	N/A	N/A	IP WB
13-2092	Biotin anti-mouse CIRE (DC-SIGN, CD209)	N/A	N/A	FC
11-2092	Fluorescein isothiocyanate (FITC) anti-mouse CIRE (DC-SIGN, CD209)	488	518	FC
12-2092	Phycoerythrin (PE) anti-mouse CIRE (DC-SIGN, CD209)	488	575	FC
17-2092	Allophycocyanin (APC) anti-mouse CIRE (DC-SIGN, CD209)	633	660	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209). CIRE/DC-SIGN was identified by its expression on CD8α- dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN. Human DC-SIGN was originally identified in human placenta for its ability to bind the HIV envelope protein gp120 in a CD4-independent manner. CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein. It contains a C-terminal, extracellular, Carbohydrate Recognition Domain (CRD) that is predicted to bind mannose and other carbohydrates in a Ca²⁺ dependent manner. It has been postulated that CIRE/DC-SIGN may play a role in T-dendritic cell interactions through binding with members of the ICAM family. CIRE/DC-SIGN is differentially expressed by sub-populations of dendritic cells and preliminary data suggest that its expression varies depending on the activation state of the host. CIRE/DC-SIGN is down-regulated in spleen-derived dendritic cell cultures supplemented with GM-CSF. While human DC-SIGN is predominantly expressed in dendritic cells, CIRE/DC-SIGN mRNA has also been detected in B cells. The LWC06 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This LWC06 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This LWC06 antibody has been tested by flow cytometric analysis of mouse CIRE/DC-SIGN-transfected CHO cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Caminschi I, Lucas KM, O’Keeffe MA, Hochrein H, Laabi Y, Brodnicki TC, Lew AM, Shortman K, and Wright MD. 2001. Molecular cloning of a C-type lectin superfamily protein differentially expressed by CD8α^- splenic dendritic cells. Mol Immunol. 38: 365-373.

O’Keeffe M, Hochrein H, Vremec D, Caminschi I, Miller JL, Anders EM, Wu L, Lahoud MH, Henri S, Scott B, Hertzog P, Tatarczuch L, and Shortman K. 2002. Mouse plasmacytoid cells: long-lived cells, heterogeneous in surface phenotype and function, that differentiate into CD8⁺ dendritic cells only after microbial stimulus.

Park CG, Takahara K, Umemoto E, Yashima Y, Matsubara K, Matsuda Y, Clausen BE, Inaba K, and Steinman RM. 2001. Five mouse homologues of the human dendritic cell C-type lectin, DC-SIGN. 13(10): 1283-1290.

Caminschi I, Corbett AJ, Zahra C, Lahoud M, Lucas KM, Sofi M, Vremec D, Gramberg T, Pohlmann S, Curtis J, Handman E, van Dommelen SL, Fleming P, Degli-Esposti MA, Shortman K, Wright MD. Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int Immunol. 2006 May;18(5):741-53. (LWC06, WB, PubMed)

Related Products

Cat. 12-2091  Phycoerythrin (PE) anti-mouse CIRE (DC-SIGN, CD209) (clone 5H10)
Cat. 13-2091  Biotin anti-mouse CIRE (DC-SIGN, CD209) (clone 5H10)
Cat. 14-2091  Affinity Purified anti-mouse CIRE (DC-SIGN, CD209) (clone 5H10)
Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4321  Biotin Rat IgG2a Isotype Control