Staining of human peripheral blood lymphocytes (left) and mature dendritic cells (right) with 0.015 μg of Biotin Mouse IgG2b Iso Cntrl (cat. 13-4732) (open histogram) or 0.015 μg of Biotin MG38 (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.
Contents: Biotin anti-human CD205 (DEC-205) Catalog Number: 13-2059 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: MG38 Host/Isotype: Mouse IgG2b, κ HLDA Workshop: N/A Storage Conditions: Store at 4°C. DO NOT FREEZE.
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Cat. No.
Size
Price
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13-2059-80
25 ug
13-2059-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor. CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo. CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8+ DCs that are believed to play a role in cross-priming. Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC). All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MG38 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood leukocytes. This can be used at less than or equal to 0.03 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hawiger D, Inaba K, Dorsett Y, Guo M, Mahnke K, Rivera M, Ravetch JV, Steinman RM, Nussenzweig MC. (2001) Dendritic cells induce peripheral T cell unresponsiveness under steady state conditions in vivo. J Exp Med. Sep 17;194(6):769-79.
Bonifaz L, Bonnyay D, Mahnke K, Rivera M, Nussenzweig MC, Steinman RM. (2002) Efficient targeting of protein antigen to the dendritic cell receptor DEC-205 in the steady state leads to antigen presentation on major histocompatibility complex class I products and peripheral CD8+ T cell tolerance. J Exp Med. Dec 16;196(12):1627-38.
Jiang W, Swiggard WJ, Heufler C, Peng M, Mirza A, Steinman RM, Nussenzweig MC. (1995) The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature. May 11;375(6527):151-5.
Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM. 2000. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum Immunol. 61(8):729-38.
