Mouse bone marrow cells were stained with staining buffer (autofluorescence) (left) or Anti-Mouse CD201 (EPCR) Biotin (right), followed by Streptavidin Alexa Fluor 594, and Anti-Mouse CD34 FITC (cat. 11-0341), Anti-Mouse Ly-6A/E (Sca-1) PE (cat. 12-5981), Anti-Mouse CD117 (c-Kit) PE-Cy7 (cat. 25-1172), and anti-mouse lineage APC. Events displayed are Sca-1+, c-kit+ and Lineage-. Images provided courtesy of Hiromitsu Nakauchi.
Contents: Anti-Mouse CD201 (EPCR) Biotin Catalog Number: 13-2012 Concentration: 0.5 mg/ml Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Clone: eBio1560 (1560) Host/Isotype: Rat IgG2b, κ
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13-2012-80
25 ug
13-2012-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis.
Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.
Applications Tested
This eBio1560 (1560) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest, particularly when analyzing expression on endothelial cells where CD201 expression is very high.
References
Balazs AB, Fabian AJ, Esmon CT, Mulligan RC.
Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow.
Blood. 2006 Mar 15;107(6):2317-21. Epub 2005 Nov 22. (1560, FC, FA, PubMed)
Fukudome K, Esmon CT.
Molecular cloning and expression of murine and bovine endothelial cell protein C/activated protein C receptor (EPCR). The structural and functional conservation in human, bovine, and murine EPCR.
J Biol Chem. 1995 Mar 10;270(10):5571-7.
Li W, Zheng X, Gu JM, Ferrell GL, Brady M, Esmon NL, Esmon CT.
Extraembryonic expression of EPCR is essential for embryonic viability.
Blood. 2005 Oct 15;106(8):2716-22.
